Education
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1990-1992
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Chemistry degree. Franche-comte University, Besancon, France.
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1992
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Chemistry degree. Franche-comte University, Besancon, France.
BS Physical Chemistry. Louis Pasteur University, Strasbourg, France.
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1993
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MS Physical Chemistry. Louis Pasteur University, Strasbourg, France.
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1996
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Ph.D. Physical Chemistry, Polymers, Institut Charles Sadron,
Strasbourg, France. Passed with distinction.
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Experience
2006-present: Assistant Professor.
SUNY at Stony Brook.
Department of Materials Science.
Program of Chemical Engineering.
2002- August 2006:
Adjunct Professor/Research Scientist.
SUNY at Stony Brook.
Department of Materials Science.
- Control of protein organization on synthetic surfaces and subsequent control of cell behavior.
- Cell mechanical properties related to surface mechanical properties.
- Cell response to chemistry.
- Cell response to nano- and micro-scale patterns, as well as nano- and microparticles.
- Extracellular matrix on surfaces: lab on a chip ? comparison between normal extracellular matrix formation to pathological extracellular matrix, such as diabetes.
1998- 2002:
Research Scientist.
SUNY at Stony Brook.
Department of Orthopaedics-Biomedical Engineering with Prof. Kenneth McLeod.
- Protein adsorption and cell adhesion on surfaces. Extracellular matrix organization.
- Engineered novel surfaces to mimic cell membrane: Influence of surface charge density protein adsorption.
- Influence from external agents (chemicals or electrical)
- Effect on cell adhesion.
- Analysis of protein structure and organization by Atomic Force Microscopy in situ.
1998- present:
Brookhaven National Laboratory Guest
1997- 1998:
Postdoctorate.
SUNY at Stony Brook
Department of Chemistry in Prof. Ben Chu's laboratory.
- Design of novel surfaces useful for DNA separation during electrophoresis
- Surface DNA Electrophoresis
- Nanopatterning with copolymers.
- Surface Interactions with DNA.
- Surfaces characterization by AFM, DNA adsorption (Quartz Crystal Microbalance).
- DNA dynamics by confocal microscopy using laser induced Fluorescence.
Techniques
- AFM (Atomic Force Microscopy). LFM (Lateral Force Modulation).
- Confocal microscopy.
- Cell cultures.
- Surface preparation.
- FRAP (Fluorescence Recovery After Photobleaching), light scattering, electric birefringence.
- Electrophoresis, capillary electrophoresis.
- HPLC (chromatography), ellipsometry, UV- spectroscopy.
- Langmuir/Blodgett films.
- Experience in neutron scattering, FTIR, scientific and graphic softwares.
Patents
- Adsorption of Polyampholytes to Charged Surfaces and Assays.
- Patent filed on Jan.13th, 2006. Single cancer cell detection.
Grants
National Science Foundation/NER:
"Nano-Fabricated Photosensitive Polymers for Controlled Cell Manipulation in 2D & 3D".
Award number: 30302
Award Period: 9/1/03- 8/31/05.
Nan-Loh Yang, CUNY and Nadine Pernodet, SUNY. $100,000.
National Institute of Health/NIBIB.
"Physics and Biology - A Material Sciences Approach".
Miriam Rafailovich and Nadine Pernodet. $10,000. 1R13 EB004243-01.
Award number: 32828.
Support for Conference held in Paris from June 28 to July 1st, 2004.
2004 USB-BNL Seed Grant
"Which Comes Frist, the Eggshell or Its Genes?
Mimicking Biomineralization with Artificial Protein Networks".
Miriam Rafailovich, Elaine DiMasi and Nadine Pernodet. $20,000.
Award number: 000079.
Conference Organization
"Physics and Biology: A Materials Approach".
Organizer Committee: Nadine Pernodet, Miriam Rafailovich, Pascal Silberzan.
Sponsored by NSF and NIH.
Honors
- Doctorate Fellowship from the French Research and Technology Agency.
- Mentor Recognition. Siemens Westinghouse Competition. Siemens Foundation.
Membership
- 2003- present: American Physical Society, APS.
- 2003- present: Materials Research Society, MRS.
Judge for LISEF (Long Island Science and Engineering Fair) fair 2005 for High School students.
Publications
- B.Tinland, N.Pernodet, G.Weill (1996). "Field and pore size dependence of the electrophoretic mobility of DNA: a combination of FRAP and Electric Birefringence measurements", Electrophoresis, 17, 1046-1051.
- N.Pernodet, M.Maaloum, B.Tinland, (1997). "Pore size of agarose gels by Atomic Force Microscopy", Electrophoresis, 18, 55-58.
- N. Pernodet, B. Tinland, (1997). "Influence of *-DNA concentration on mobilities and dispersion coefficients during agarose gel electrophoresis", Biopolymers, 471-478.
- M. Maaloum, N. Pernodet, B. Tinland (1998). "Agarose Gel Structure using Atomic Force Microscopy. Gel concentration and ionic strength effects", Electrophoresis, 19, 1606-1610.
- B. Tinland, N. Pernodet, A. Pluen (1998). "Band broadening in gel electrophoresis: Scaling laws for the dispersion coefficient measured by FRAP", Biopolymers, 46, 201-214.
- N. Pernodet, B. Tinland, J. Sturm, G. Weill (1999). "Brownian diffusion and electrophoretic transport of double-stranded DNA in agarose gels", Biopolymers, 50: (1), 45-59.
- N. Pernodet, K. Shin, H. White, A. Rahman, C.J. Durning, J. Sokolov, M. Rafailovich, B. Chu (2006) "The Effect of Surface Interactions on DNA Adsorption", Langmuir, (to be submitted).
- N. Pernodet, K. Shin, D. Gersappe, J. Sokolov, M. Rafailovich, B. Chu (2000). "DNA Electrophoresis on a Surface", Physical Review Letters, 85(26), 5651-5654.
- N. Pernodet, M. Rafailovich, J. Sokolov, D. Xu, N.L. Yang, K.J. McLeod (2003). "Surface charge density mediates spontaneous fibronectin matrix formation". J.Biomed.Mat.Res. 64(4), 684-692.
- N. Pernodet, L. Collazo, M. Rafailovich, J. Sokolov, D. Xu, N.L. Yang, K.J. McLeod (2006). "Electric Field Effects on Fibronectin Matrix Formation and Cell Behavior", American Journal of Physiology- Cell Physiology, (submitted).
- J. Jerome, S. Zhu, Y-S. Seo, M. Ho, N. Pernodet, R. Gambino, J. Sokolov, M. Rafailovich, V. Zaitsev, S. Schwartz, R. DiNardo (2004). "Phase Segregation of Thin Film Polymer Blends on Au Nanopatterned Si Substrates". Macromolecules 37(17), 6504-6510.
- N. Pernodet, Xiaohua Fang, Asya Bakhtina, Abraham Ulman, Miriam Rafailovich (2006). "Effects of Citrate/Gold Nanoparticles on Human Dermal Fibroblasts".
SMALL 2(6), 766-773.
- K. Subburaman, N. Pernodet, S.Y. Kwak, E. DiMasi, S. Ge, V. Zaitsev, X. Ba, N.L. Yang, M. Rafailovich. (2006) " Templated Biomineralization on Self-Assembled Protein Fibers" PNAS 2006 (published).
- N. Pernodet, Jessica Fields, Kaustabh Ghosh, Taylor Bernheim, Shouren Ge, Lenny Slutsky, Jonathan Sokolov, Miriam Rafailovich. (2006). "Single Cancer Cell Detection through Mechanics". Nature Biotechnology (submitted).
Conferences and Proceedings/Abstracts
- APS March Meeting, March 2006, Baltimore.
- MRS Meeting, November 2005, Boston.
"Single cancer cell detection"
Nadine Pernodet, Jessica Fields, Lenny Slutsky, Kaustabh Ghosh, Taylor Bernheim, Shouren Ge, Jonathan Sokolov, Miriam Rafailovich.
- MRS Meeting, November 2005, Boston.
"Effects of Aging and Cell Mechanical Properties"
Nadine Pernodet, Lenny Slustsky, Anna Shneidman, Madelyn Ho, Shouren Ge, Miriam Rafailovich.
- MRS Meeting, November 2005, Boston.
"Biomineralization Studies on Self-Assembled Protein Fibers"
Karthikeyan Subburaman, Nadine Pernodet, Seo-Young Kwak, Elaine DiMasi, Shouren Ge, Nan-loh Yang, Miriam Rafailovich.
- MRS Meeting. November 2004. Boston.
"Effects of Gold Nanoparticles on Dermal Fibroblasts"
Nadine Pernodet, Xiaohua Fang, Asya Bakhtina, Abraham Ulman, Miriam Rafailovich.
- MRS Meeting. November 2004. Boston.
"Protein Self-Assembly on Patterned Surfaces"
Karthikeyan Subburaman, Nadine Pernodet, Miriam Rafailovich, Nan-Loh Yang.
- Physics and Biology: A Materials Approach. NSF/NIH. June 2004. Paris, France.
"Control of Cell Morphology through Protein Organization on Au/Si Micropatterns"
Nadine Pernodet, Lenny Slutsky, John Jerome, Miriam Rafailovich.
- American Physical Society. March Meeting 2004. Montreal, Canada.
"Control of cell morphology through protein organization on Au/Si micropatterns"
Nadine Pernodet, Lenny Slutsky, John Jerome, Miriam Rafailovich.
- American Physical Society. March Meeting 2004. Montreal, Canada.
"Cell Responses to Patterned Surfaces from Micro- to Nano-scale topography"
Madelyn Ho, Lenny Slutsky, Miriam Rafailovich, Nadine Pernodet.
- American Physical Society. March Meeting 2004. Montreal, Canada.
"ECM Proteins Glycosylation and Relation to Diabetes"
Nadine Pernodet, Ayla Bloomberg, Vandana Sood, Lenny Slutsky, Shouren Ge, Richard Clark, Miriam Rafailovich.
- NSF/MRSEC Workshop. SUNY at Stony Brook. 2003.
"Self-Organization of Proteins and Cells on Surfaces".
Nadine Pernodet, Miriam Rafailovich, Richard Clark, Elena Cimboiasu,, Nan-Loh Yang, Lenny Slutsky, Madelyn Ho, Michelle Simpser, Rikky Frenkel, John Jerome.
- Workshop NSF/MRSEC. April 2003. Stanford University.
"Self-Organization of Proteins on Patterned Surfaces- (A Seed Project of the GARCIA MRSEC)"
Nadine Pernodet, John Sokolov, Miriam Rafailovich, Richard Clark, Lourdes Collazo, Elena Cimboiasu,, Nan-Loh Yang, Lenny Slutsky, Madelyn Ho, Michelle Simpser, Rikky Frenkel.
- American Physical Society. March Meeting 2003. Austin, TX.
"Control of fibronectin organization by Au/Si micropatterns and impact on cell morphology".
N. Pernodet, L. Slutsky, J. Jerome, M. Rafailovich.
- American Physical Society. March Meeting 2003. Austin, TX.
"Spontaneous Natural Fibrillogenesis on Charged Surfaces".
N. Pernodet, M. Simpser, R. Frenkel, L. Collazo, M. Rafailovich.
- Functional Nanostructure Symposium, August 2001. Brookhaven National Lab. Upton, NY.
"Self-Organization of Fibronectin on Sulfonated Polystyrene"
Nadine Pernodet, Nan-Loh Yang, Miriam Rafailovich, Kenneth McLeod.
- BEMS 23rd annual meeting, Saint Paul, Minnesota, June 10-14, 2001:
"Induced Charge Effects on Extracellular matrix formation"
N. Pernodet, C. Laramee, M. Rafailovich, J. Sokolov, K. McLeod.
- The 4th Annual University/Industry Workshop GARCIA-MRSEC:
Biofunctional Systems and Polymers at Interfaces, Munich, Germany, June 20-22, 2001:
"Spontaneous Fibronectin Matrix Organization Induced by Charged Surfaces"
N. Pernodet, M. Rafailovich, J. Sokolov, K. McLeod.
- BEMS 22nd annual meeting, Munich, Germany, June 9-16, 2000:
"Surface Charge Density Mediates Spontaneous Fibronectin Matrix Formation in the Absence of Cells"
N. Pernodet, M. Rafailovich, J. Sokolov, K. McLeod.
- American Physical Society, March 2000 meeting, Mineapolis, Minnesota, March 20-24, 2000.
Section: Biopolymers. Ref: K23.013
"Spontaneous formation of fibronectin network on charged surfaces".
N. Pernodet, D. Gersappe, J. Sokolov, M. Rafailovich, K. McLeod.
- American Physical Society Centennial Meeting, Atlanta, Georgia, March 20-26, 1999.
"DNA Fractionation on a Silicon Wafer".
N. Pernodet, K.W. Shin, H. White, J. Sokolov, M. Rafailovich, B. Chu.
- MRSEC, Stony Brook, New York, 1998.
"DNA Electrophoresis on a block copolymer Surface".
N. Pernodet, K.W. Shin, H. White, M. Rafailovich, J. Sokolov, B. Chu.
- Garcia MRSEC Annual Workshop, Brookhaven National Laboratory, October 25-27, 1998.
"The Effect of Surface Interaction on DNA Adsorption and DNA Fractionation".
N. Pernodet, K.W. Shin, H. White, A. Rahman, C. Durning, M. Rafailovich, J. Sokolov, B. Chu.
- Garcia MRSEC Annual Workshop, Brookhaven National Laboratory, October 25-27, 1998.
"Protein Adsorption on Polystyrene Surface and Sulfonated Polystyrene Surfaces".
N. Pernodet, M. Rafailovich, J. Sokolov, K. McLeod.
Please look at the faculty and
facilities
of the Stony Brook Materials Department.
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